Not all lash serums are created equal. We tested the internet’s most-hyped formulas, tracked our results, and asked ophthalmologist Michael Brinkenhoff, M.D., founder of RevitaLash Cosmetics, to clarify the science behind lash growth. Below: the real answers to the most-Googled questions, including how long it takes to see results, what “works” actually means, and whether lash serums are safe for sensitive eyes, extensions or pregnancy
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Do lash serums actually work?
That depends on the product... And your expectations. Most eyelash serums on the market are cosmetic, not pharmaceutical. They’re designed to condition the lashes and support retention, often using peptides, amino acids and humectants. “They’re developed to improve the appearance of natural lashes,” explains Dr. Michael Brinkenhoff, founder of RevitaLash. In other words, if you're expecting new lashes to suddenly sprout, you may be disappointed. But if you want your existing lashes to look healthier and stay in place longer, a good lash serum can deliver visible results (provided you use it correctly and consistently).
How long before I see results?
The lash growth cycle spans several weeks, so results are gradual. “People often expect results within days, but that’s just not how hair growth works,” notes Dr. Brinkenhoff. In a six-week consumer study of RevitaLash Advanced, 98 per cent of users reported improved lash appearance. With steady, once-daily use, most cosmetic serums deliver noticeable results in eight to ten weeks. If nothing changes after that, it’s either the wrong formula or you’re not using it consistently enough.
What’s the right way to apply eyelash serum?
Application isn’t complicated, but it’s where most people go wrong. “A little goes a long way,” says Brinkenhoff. “Use one dip of the applicator for both eyes, and draw a thin line just above the lash line on clean, dry skin.” You don’t need to soak the lashes themselves and avoid the lower lash line entirely, as product transfer can increase the risk of irritation. Finally, wait at least two to three minutes before applying skincare or mascara to give the formula time to absorb. And no, applying more than once a day won’t accelerate results. It’s just a shortcut to red, itchy lids.
Are eyelash serums safe?
In most cases, yes. But not always. While Nordic regulations require that cosmetic products are safe for their intended use, that doesn’t mean every serum works for every eye. Potential side effects include redness, itching, and skin discolouration along the lash line. Serums with prostaglandin analogues (often marketed as high-performance) are more likely to cause irritation. If you wear contact lenses, have had eye surgery, or experience chronic dryness, speak to an eye specialist before introducing a lash serum.
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Can I use lash serum with extensions?
Yes, though not every formula plays well with glue. If you wear lash extensions, look for oil-free, water-based serums specifically marked as compatible. “RevitaLash Advanced is safe to use with lash extensions,” says Brinkenhoff. “The oil-free formula won’t compromise the bond of the adhesive.” Still, always check with your lash technician as some recommend pausing serum use for the first 48 hours after a fill to avoid premature shedding.
Bonus tip: a conditioning serum can help maintain the health of your natural lashes underneath the extensions, potentially making fills last longer.
Is it safe to use lash serums during pregnancy or when breastfeeding?
This is where blanket advice stops. “We do not recommend using our products if you are pregnant or nursing,” says Brinkenhoff. While there are no definitive studies proving harm, most brands err on the side of caution and advise discontinuation. Hormonal fluctuations during pregnancy can also change how your skin reacts to products, making irritation more likely. If you’re unsure, consult your doctor before starting any lash serum while pregnant or breastfeeding.
What if my eyes are sensitive?
Sensitivity doesn’t rule you out, but it does narrow the playing field. Look for fragrance-free, alcohol-free formulas made without prostaglandin analogues – these tend to be the most irritating ingredients. Brinkenhoff notes that RevitaLash created Advanced Sensitive with this group in mind: “It uses a liposomal delivery system to release ingredients slowly and more gently.” Even so, start slowly (every other night at first), and stop immediately if you notice stinging, redness, or flaking at the lash line. As with any eye-area product, patch-testing is a non-negotiable.
Do results last if I stop using it?
No. And that’s not a fault of the product, but a function of biology. “Results are not permanent,” confirms Brinkenhoff. “When you discontinue use, your lashes will return to their original appearance over time.” Like skincare or haircare, lash serums require consistency to maintain results. Once you stop, the lashes grown or retained during treatment will eventually shed as part of the natural cycle, and new ones will grow in unaffected.
What should I look for in a lash serum?
More isn’t always better. The best formulas keep things simple: peptides, humectants, panthenol or biotin for conditioning, and a precise, easy-to-use applicator. “Look for safety, science and user experience,” says Brinkenhoff, meaning formulas backed by clinical testing, brands that are transparent about ingredients, and instructions that don’t require guesswork. If the label overpromises or the ingredient list is vague, keep looking.
The 10 best eyelash serums to buy in 2025:
Best overall eyelash serum
What it is: This formula is still the lash serum gold standard. Developed by an ophthalmologist and worn by basically every beauty editor worth her spoolie, RevitaLash delivers the kind of lash improvement that builds slowly and sticks around.
Why we love it: It may not be a flashy transformation, but you're guaranteed a subtle, steady one. Over six to eight weeks, lashes look glossier, stronger, and are better able to hold a curl. It dries quickly, doesn’t interfere with makeup or extensions, and the applicator delivers exactly what’s needed — no more, no less. If you’re new to lash serums and just want something that works without side effects or surprises, start here.
Best affordable eyelash serum
What it is: A peptide-powered lash and brow serum that does the basics well: hydration, retention, and gentle support. And all without prostaglandins or fragrance.
Why we love it: No bells, no whistles, no promises it can’t keep. This under-20€ tube is a smart starting point if you're serum-curious but sceptical. The brush is straightforward, the formula is light, and over time, lashes do look slightly more groomed and resilient. Also good for treating those overplucked brows.
Best eyelash serum for growth and length
What it is: This is one of the few mass-market serums that includes a prostaglandin analogue — the ingredient class known to actually extend the growth phase of lashes.
Why we love it: If you’re after more length than volume, this one tends to deliver faster than others. Our testers saw visible change around the six-week mark. But it comes with a caveat: prostaglandin analogues can cause irritation in sensitive eyes, so patch test before you commit. It’s not for everyone, but for some, it’s the difference between “natural” and “wait, are you wearing extensions?”
Best eyelash serum for fullness and thickness
What it is: A Swedish-developed serum focused on strengthening and reinforcing the lashes you already have. Vegan, prostaglandin-free, and made with biotin, hyaluronic acid, and peptides.
Why we love it: Where RapidLash pushes growth, Sweed is more about density. After a few weeks, lashes feel sturdier, shed less, and look more plush even without mascara. The short ingredient list avoids common irritants, making it a safe pick if your focus is on long-term conditioning rather than dramatic results.
Best prostaglandin-free eyelash serum
What it is: A clean-lean Scandinavian lash serum formulated without prostaglandin analogues, instead relying on peptides and plant-based ingredients to strengthen lashes over time.
Why we love it: This is the lash serum for people who’ve heeded the warnings, read the forums, and decided they’re not interested in rolling the dice. It’s gentle, fragrance-free, and non-irritating making it a solid low-risk option for anyone who wants better lashes without worrying about lid discolouration or sensitivity.
Best oil-free serum for lash extensions
What it is: An oil-free, water-based lash serum that pairs well with lash extensions... and happens to be one of the most searched-for formulas online.
Why we love it: This serum sits in the sweet spot between performance and practicality. It’s powerful (contains a prostaglandin analogue), but still safe to use with extensions, and is often recommended by lash techs as a maintenance serum between fills. Expect a difference in length and curl if used consistently, but skip this one if your eyes are on the reactive side.
Best eyelash serum for sensitive eyes
What it is: A prostaglandin-free formula developed specifically for reactive eyes. Ophthalmologist-tested and hypoallergenic.
Why we love it: Many serums claim to be “gentle”, but this one actually is. If your eyes sting after micellar water, or if you’ve given up on lash serums altogether, this could be your re-entry point. It won’t give you dramatic length, but it will quietly improve lash quality over time, all without triggering a flare-up.
Best pregnancy-safe eyelash serum
What it is: A prostaglandin-free lash serum from the bond-building brand best known for rescuing bleached hair.
Why we love it: No lash serum is officially certified for pregnancy, but this one skips all the usual red-flag ingredients and has a short, transparent ingredient list. The formula includes biotin, peptides and panthenol (all solid lash conditioners) and gets bonus points for the brand’s commitment to testing and user safety. As always, check with your doctor if you’re unsure.
Best dual-use lash and brow serum
What it is: A clean, two-in-one serum powered by hemp seed extract and peptides, made for anyone whose lashes and brows are equally neglected.
Why we love it: Convenient, quick-drying, and genuinely effective on both lash and brow. The wand makes it easy to apply exactly where it’s needed (especially on sparse brows), and the lightweight texture doesn’t interfere with makeup. A good pick for minimalists or anyone trying to get more out of fewer products.
What it is: A prestige lash-and-brow serum powered by the brand’s TFC8® complex, typically found in their €200+ face creams.
Why we love it: This is luxury care for your lash line. It doesn’t promise dramatic results, but it does deliver healthier, softer, more conditioned lashes and brows over time. If you're already a Bader devotee, this fits seamlessly into your routine. If you're not, it’s still one of the most luxurious serums on the market: silky texture, beautiful applicator, and a freakishly good formulation.